3D Cell Culture 2014 - Advanced Model Systems, Applications & Enabling Technologies

Lecture Programme

(subject to change)

Wednesday, 25 June 2014
Thursday, 26 June 2014
Friday, 27 June 2014


Wednesday, 25 June 2014

11:00   Welcome

Prediction in cancer research
Chair   T. Noll, University of Bielefeld/D

11:05   Keynote Lecture
             IMI PREDECT: Characterising 3D models for target validation in cancer
            J.A. Hickman, Institut de Recherches Servier, Paris/F

11:50   Identification of genetic determinants for tumor spheroid morphology and drug

             M.C. Park, S. Kim, Seoul National University/ROK

12:10   Predicting clinical therapeutic response in primary ovarian cancer using human
             cancer tissue-originated spheroids

             O. Hoffmann, Spherotec GmbH, Martinsried/D; R. Loewe, GeneWake GmbH, Munich/D;
             D. Beer, University Hospital LMU, Munich/D; H. Jansen, University Hospital TUM,
             Munich/D; B. Mayer, Spherotec GmbH, Martinsried/D

12:30   A combined in silico and in vitro lung cancer model on a decellularized matrix for
             individualized drug response predictions

             A.T. Stratmann, D. Fecher, University Hospital of Würzburg/D; G. Wangorsch,
             University of Würzburg/D; C. Göttlich, T. Walles, H. Walles, University Hospital of
             Würzburg/D; T. Dandekar, University of Würzburg/D; G. Dandekar, S.L. Nietzer,
             University Hospital of Würzburg/D

12:50   Lunch

Assessment of drug safety and toxicity
Chair  J. Kelm, InSphero AG, Schlieren/CH

14:15   Keynote Lecture
             Application of 3-dimensional co-culture models in drug safety assessment
             A.B. Roth, Hoffmann-La Roche AG, Basel/CH

15:00   Enabling in vivo relevant drug toxicity testing in organ like cell culture models
J. Schütte, S. Werner, S. Hajek, NMI Natural and Medical Sciences Institute, Reutlingen/D;
             J. Böttger, R. Gebhardt, University Leipzig/D; S. Maier, D. Stoll, M. Stelzle, NMI Natural and
             Medical Sciences Institute, Reutlingen/D

15:20   Validation of in vitro cell health assays for 3D microtissues
T.L. Riss, M.P. Valley, C.A. Zimprich, K.R. Kupcho, D.F. Lazar, Promega Corporation,
             Madison, WI/USA

15:40   New 3D cell model as an alternative for animal testing in osteoarthritis research
I. Ponomarev, fzmb GmbH, Bad Langensalza/D; A. Lang, BSRT / Charité, Berlin/D;
             T. Reuter, fzmb GmbH, Bad Langensalza/D

15:50   A multifunctional device for automated 3D-cultivation of hepatocytes and monitoring
             of drug toxicity based on a novel flow-through ELISA

             D. Kuersten, M. Baca, J. Hampl, U. Fernekorn, M. Klett, A. Läffert, K. Friedel, A. Schober,
             Ilmenau University of Technology/D

16:00   Coffee Break

Compound screening and profiling
Chair   C. Burger, Merck KGaA, Darmstadt/D

16:30   Keynote Lecture
             Ultra-high content analysis of 3D cell cultures for compound screening and profiling
             L. Price, OcellO B.V., Leiden/NL

17:15   3D microtissues for the discovery of chemoradiation-sensitizing compounds
             N. Anastasov, I. Hoefig, V. Radulovic, Helmholtz Zentrum München, Neuherberg/D;
             S. Stroebel, InSphero AG, Schlieren/CH; C. Thirion, Sirion Biotech GmbH, Martinsried/D;
             J. Lichtenberg, J. Kelm, InSphero AG, Schlieren/CH; J.M. Atkinson, Helmholtz Zentrum
             München, Neuherberg/D

17:35   Microfluidic 3D cell culture platform for high-throughput drug screening
             S. Trietsch, MIMETAS B.V., Leiden/NL; V. van Duinen, Leiden University/NL; J. Joore,
             MIMETAS B.V., Leiden/NL; T. Hankemeier, Leiden University/NL; P. Vulto, MIMETAS B.V.,

17:55   Multiplexing resazurin reduction, spheroid volume and acid phosphatase assays
             for spheroid viability in high-throughput

             D.P. Ivanov, T. Parker, D. Walker, C. Alexander, M.C. Garnett, University of Nottingham/UK;
             M. Ashford, P. Gellert, AstraZeneca, Macclesfield/UK

18:15   Human organotypic test models for functional, immuno-pharmacological drug

             M. Schmolz, EDI GmbH, Reutlingen/D

18:25   End of Lecture Programme of Day 1

18:30-19:30 Section Member Assembly

18:30-21:00 Poster Session / Poster Party


Thursday, 26 June 2014

Disease models
Chair   T. May, InSCREENeX GmbH, Braunschweig/D

09:00   Keynote Lecture
             3D biopatterning technologies to engineer model systems for cancer and neural

              U. Demirci, S. Guven, Harvard Medical School, Cambridge, MA/USA

09:45   Studying gene function analysis in 3D tumor microtissue models
             S. Stroebel, InSphero AG, Schlieren/CH; C.R. Thoma, N. Rösch, B. Calpe, W. Krek,
             ETH Zurich/CH; J.M. Kelm, InSphero AG, Schlieren/CH

09:55   Perfusion induced chemoresistance increase in multicellular pleural cancer
             spheroids cultured on a microfluidic chip

             J. Ruppen, E. Marconi, University of Bern/CH; R. Schmid, R. Peng, T.M. Marti, University
             Hospital of Bern/CH; O. Guenat, University of Bern/CH

10:05   Human 3D osteosarcoma models for drug testing
             M. Rimann, S. Laternser, Zurich University of Applied Sciences, Wädenswil/CH;
             J.M. Kelm, InSphero AG, Schlieren/CH; A. Gvozdenovic, R. Muff, B. Fuchs, University
             Hospital Balgrist, Zurich/CH; U. Graf-Hausner, Zurich University of Applied Sciences,

10:15   A novel 3D human airway model for oncology research
             C. Mas, S. Huang, L. Wisniewski, S. Constant, OncoTheis, Geneve/CH

10:25   Coffee Break

Engineering of 3D models
Chair   U. Graf-Hausner, Zurich University of Applied Sciences, Wädenswil/CH

11:00   Keynote Lecture
             Development and application of alternative methods in the chemical industry
             T. Ramirez, BASF SE, Ludwigshafen/D

11:45   Laser-assisted bioprinting (LaBP) as a sophisticated means to create a
             three-dimensional skin equivalent

             S. Michael, H. Sorg, Hannover Medical School/D; L. Koch, Laser Centre Hannover/D;
             C.T. Peck, Hannover Medical School/D; A. Deiwick, Laser Centre Hannover/D; V. Coger,
             Hannover Medical School/D; B. Chichkov, Laser Centre Hannover/D; P.M. Vogt,
             K. Reimers, Hannover Medical School/D

12:05   A 3D skin sensitization model: KeratinoSens cells in RHE
             S. Mathes, B. Striegl, Zurich University of Applied Sciences, Wädenswil/CH; A. Natsch,
             R. Emter, T. Haupt, Givaudan, Dübendorf/CH; P. Girling, CELLnTEC, Bern/CH;
             U. Graf-Hausner, Zurich University of Applied Sciences, Wädenswil/CH

12:15   An innovative bio-printing platform to engineer lung tissue
             L. Horvath, University of Fribourg, Marly/CH; M. Thurner, regenHU Ltd.,
             Villaz St. Pierre/CH; C. Jud, A. Fink, B. Rothen-Rutishauser, University of Fribourg,

12:25   Differentiated and reproducible cell models of the airway epithelium through
             computer-controlled air-liquid interface cultivation

             M. Aufderheide, J. Rach, Cultex Laboratories GmbH, Hannover/D

12:35   High-throughput manufacturing of human epidermal models
             A. Traube, M. Walter, L. Schober, Fraunhofer IPA, Stuttgart/D; F. Groeber, H. Walles,
             Fraunhofer IGB, Stuttgart/D; K.R. Mewes, Henkel AG & Co. KGaA, Düsseldorf/D;
             A. Traube, Fraunhofer IPA, Stuttgart/D

12.45   Lunch

From stem cells and primary cells to disease models
Chair   H. Hauser, Helmholtz Centre for Infection Research, Braunschweig/D

14:00   Keynote Lecture
             Human pluripotent stem cells: cardiac and vascular disease in a dish
             C.L. Mummery, Leiden University Medical Center/NL

14:45   Characterization of single and co-cultured human iPSC-derived cardiomyocytes and
             cardiac fibroblasts in scaffold-free 3D microtissues

             P. Beauchamp, Bern University/CH; W. Moritz, I. Agarkova, J. Kelm, InSphero AG,
             Schlieren/CH; N. Ullrich, Bern University/CH; T.M. Suter, C. Zuppinger, Bern University

15:05   Towards improved predictability in pre-clinical research: human dopaminergic 3D in
              vitro model for development of gene delivery strategies

              D. Simão, C. Pinto, ITQB-UNL / IBET, Oeiras/P; S. Piersanti, Biomedical Science Park
              San Raffaele / Sapienza University of Rome/I; P. Fernandes, M. Serra, A.P. Teixeira,
              ITQB-UNL / IBET, Oeiras/P; A. Weston, Cancer Research UK, London/UK; S. Ibanes,
              A. Gennetier, Institut de Génétique Moléculaire de Montpellier/F; L. Collinson, Cancer
              Research UK, London/UK; I. Saggio, Biomedical Science Park San Raffaele / Sapienza
              University of Rome/I; G. Schiavo, Cancer Research UK, London/UK; E.J. Kremer,
              Institut de Génétique Moléculaire de Montpellier/F; P.M. Alves, C. Brito, ITQB-UNL / IBET,

15:25   Functional immortalized cell lines for development of bioassays in 2D and 3D
             T. May, R. Schucht, J. Zauers, InSCREENeX GmbH, Braunschweig/D; J. Schwerk,
             M. Köster, Helmholtz Centre for Infection Research, Braunschweig/D

15:35   Assessing the 3D in vitro physiology of biopsy derived primary hepatocytes
             U. Fernekorn, J. Hampl, F. Weise, M. Klett, A. Läffert, K. Friedel, A. Schober, Ilmenau
             University of Technology/D

15:45   Coffee Break

Complex 3D cultures
Chair   U. Marx, TU Berlin/D

16:15   Keynote Lecture
             Engineering of human 3D vascularised tissues including disease models
             H. Walles, University Hospital of Würzburg/D

17:00   3D angiogenesis assays in fibrin matrices
              V. Charwat, University of Natural Resources and Life Sciences, Vienna/A; P. Hofbauer,
              W. Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology,
              Vienna/A; C. Kasper, University of Natural Resources and Life Sciences, Vienna/A

17:10   Stop or go? Studying nanomaterial translocation and behavior at the human placental
             barrier using ex vivo and in vitro model systems

             T. Buerki-Thurnherr, P. Manser, Empa, St. Gallen/CH; S. Grafmüller, University Hospital
             Zurich/CH; C. Muoth, Empa, St. Gallen/CH; H. Annaheim, Zurich University of Applied
             Sciences, Wädenswil/CH; W. Jochum, P.A. Diener, Cantonal Hospital St. Gallen/CH;
             U. von Mandach, University Hospital Zurich/CH; P. Wick, Empa, St. Gallen/CH

17:30   Tooth formation in vivo and in vitro
             J. Binder, J. Bräunig, R. Lauster, M. Rosowski, TU Berlin/D

17:50   Development of stabilised aligned CNS co-culture technology to model the behaviour
             of a range of neural cell types

             C. O'Rourke, A.J. Loughlin, Open University, Milton Keynes/UK; R. Drake, TAP
             Biosystems, Royston/UK; J.B. Phillips, UCL, London/UK

18:00   End of Lecture Programme of Day 2

19:30   Conference Dinner at Greiffenegg Schlössle



Friday, 27 June 2014

Enabling technologies
Chair   R. Pörtner, Hamburg University of Technology/D

09:00   Keynote Lecture
             Rapid discovery of specific 3D culture matrixes for difficult-to-culture cell types
             M. Lütolf, EPFL, Lausanne/CH

09:45   Skin bioprinting: an approach to produce standardized skin models on demand
             M. Rimann, E. Bono, H. Annaheim, M. Bleisch, Zurich University of Applied Sciences,
             Wädenswil/CH; M. Kuster, M. Thurner, regenHU Ltd., Villaz-St-Pierre/CH; C. Meier,
             A. Bossen, Bern University of Applied Sciences, Biel/CH; U. Graf-Hausner, Zurich
             University of Applied Sciences, Wädenswil/CH

09:55   Miniaturised artificial lymph node reactor - construction and validation of a 3D cell
             culture device

             A. Böhme, L. Radke, F. Schütze, C. Rogge, T. Liebscher, A. Foitzik, M. Frohme,
             University of Applied Science Wildau/D; C. Giese, ProBioGen AG, Berlin/D

10:05   Modular segmented flow-platform for 3D cell cultivation
             K. Lemke, T. Förster, R. Römer, A. Grodrian, St. Wiedemeier, G. Gastrock, Institute for
             Bioprocessing and Analytical Measurement Techniques, Heilbad Heiligenstadt/D

10:15   Development and integration of new microfluidic building blocks for long-term 3D
             culture and analysis

             H. Kleine-Brüggeney, L. van Vliet, F. Gielen, F. Hollfelder, University of Cambridge/UK

10:25   Fabrication of 3D cell structures using self-folding polymer films
             L. Ionov, S. Zakharchenko, Leibniz-Institut für Polymerforschung Dresden e.V./D

10:35   Raman spectroscopy for marker-free 3D-tissue analysis             
             K. Schütze, S. Koch, R. Gangnus, CellTool GmbH, Bernried/D; B. Mayer, O. Hoffman,
             K. von Dehn-Rotfelser, Spherotec GmbH, Martinsried/D

10:45   Customized 3D cell culture image analysis via light sheet microscopy
             D. Haumann, T. Schenker, C. Schmidt, S. Pfeiffer, da-cons GmbH, Eggenstein-
             Leopoldshafen/D; F. Pampaloni, C. Mattheyer, E.H.K. Stelzer, BMLS Frankfurt/D

10:55   Coffee Break

Multi tissue organ systems
Chair   U. Graf-Hausner, Zurich University of Applied Sciences, Wädenswil/CH

11:25   Keynote Lecture
             Title tba
             L. Griffith, MIT, Cambridge, MA/USA

12:10   The multi-organ-chip (MOC) - a universal microfluidic platform to co-culture
             miniaturised organ equivalents at steady state

             U. Marx, TU Berlin/D; M. Busek, Fraunhofer IWS, Dresden/D; A. Lorenz, E.M. Materne,
             I. Wagner, K. Schimek, A. Ramme, TU Berlin/D; F. Sonntag, Fraunhofer IWS, Dresden/D;
            R. Horland, TU Berlin/D

12:30   Connecting hanging drops in a microfluidic network for compound testing in
             continuously perfused multi-tissue formats

             O. Frey, P.M. Misun, A. Hierlemann, ETH Zurich, Basel/CH

12:50   Survey on current development trends in in vitro drug testing in Germany and

             R. Pörtner, Hamburg University of Technology/D; I. Wagner, T. Atanasova, TU Berlin/D;
             R. Gautam, Hamburg University of Technology/D; J. Gattlen, M. Rimann, E. Bono, Zurich
             University of Applied Sciences, Wädenswil/CH; K. Rübberdt, K. Tiemann, DECHEMA e.V.,
             Frankfurt/D; U. Graf-Hausner, Zurich University of Applied Sciences, Wädenswil/CH;
             U. Marx, TU Berlin/D

13:10   End of Lecture Programme

13:10-14:00 Lunch


Lecture programme on 3D cell culture model systems, applications and enabling technologies
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