Affinity 2013

Poster Programme

The poster sessions will take place on Wednesday, 26 June at 17:10 h and on Tuesday, 27 June at 17:50 h.

Topics

1. Kinetics and thermodynamics of biomolecular interactions

P 1.01 Dansyl-ligands for FRET-based histone deacetylase assays
C. Meyners, University of Applied Systems, Darmstadt/D; M.G. Baud, M.J. Fuchter, Imperial College, London/UK; F.-J. Meyer-Almes, University of Applied Systems, Darmstadt/D
P 1.02 Thermodynamics of fluorinated benzenesulfonamides binding to carbonic anhydrase
A. Zubriene, V. Dudutiene, A. Smirnov, D. Timm, J. Gylyte, M. Kisonaite, J. Kazokaite, E. Manakova, S. Grazulis, D. Matulis, Vilnius University/LT
P 1.03 Protein-ligand affinity correlation with the ligand binding volume
V. Petrauskas, J. Gylyte, Vilnius University/LT; Z. Toleikis, University of Copenhagen/DK; P. Cimmperman, Baltic Institute of Advanced Technology, Vilnius/LT; D. Matulis, Vilnius University/LT
P 1.04 The importance of drug-target binding kinetics and energetics for drug efficacy
U. Roder, GE Healthcare Europe GmbH, Freiburg/D
P 1.05 Towards an optimized in-vitro SPR assay for antibody Fcγ receptor binding kinetics
A. Frostell, R. Karlsson, GE Healthcare Bio-Sciences AB, Uppsala/S; J. Hayes, National Institute for Bioprocessing, Research and Training, Dublin/IRL; M. Lindgren, GE Healthcare Bio-Sciences AB, Uppsala/S; P. Rudd, National Institute for Bioprocessing, Research and Training, Dublin/IRL; C. Annerén, GE Healthcare Bio-Sciences AB, Uppsala/S
P 1.06 Taking PEG solvent modulation to the next level
S. Neumann, A. Schaubmar, Roche Diagnostics GmbH, Penzberg/D
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2. Protein-protein interactions in health and disease

P 2.01 Intrinsically disordered enamel matrix protein ameloblastin and its supramolecular complexes
T. Wald, A. Osickova, R. Osicka, P. Macek, P. Sebo, O. Benada, M. Sulc, Institute of Microbiology, Prague/CZ; L. Rezabkova, Charles University, Prague/CZ; I. Slaby, Institute of Biotechnology, Prague/CZ; V. Veverka, J. Vondrasek, Institute of Organic Chemistry and Biochemistry, Prague/CZ
Cancelled by the author

P 2.02 Intracellular protein delivery using engineered nanoparticles
S.S. Soares, G.N.M. Ferreira, IBB - Institute for Biotechnology and Bioengineering, Faro/P
P 2.03 Evaluation of serum-autoantibody-biomarkers for early diagnostic testing of prostate cancer
J. Fuchs Luna, M. Gamperl, R. Kulovics, Austrian Institute of Technology, Vienna/A; P. Hofer, A. Gsur, Medical University, Vienna/A; J. Hofbauer, F. Längle, LKWN, Wiener Neustadt/A; A. Weinhäusel, Austrian Institute of Technology, Vienna/A
P 2.04 Detection of pollen allergens in the air
M.T. Thibaudon, RNSA, Brussieu/F; M.H. Hrabina, J.B. Barberon, Stallergenes, Lyon/F; K.M. Mercier, N.V. Vollmer, E.L. Ly-Morin, HORIBA Jobin Yvon S.A.S., Palaiseau/F
P 2.05 Characterization of the breast cancer marker candidate LAG3 in human plasma by hyphenated SPRi-MALDI-MS analysis
F.R. Remy-Martin, Université de Franche Comté, Besançon/F; M.E. El Osta, G.L. Lucchi, Université de Bourgogne, Dijon/F; R.Z. Zeggari, T.L. Leblois, Université de Franche Comté, Besançon/F; S.B. Bellon, E.L. Ly-Morin, C.F. Frydman, HORIBA Jobin Yvon S.A.S., Palaiseau/F; D.S. Suckau, Bruker Daltonik GmbH, Bremen/D; P.D. Ducoroy, Université de Bourgogne, Dijon/F; W.B. Boireau, Université de Franche Comté, Besançon/F
P 2.06 Horseradish peroxidase for bladder cancer treatment
O. Spadiut, Vienna University of Technology/A; L. Neutsch, F. Gabor, University of Vienna/A; C. Herwig, Vienna University of Technology/A
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4. Computational modeling for affinity maturation and drug discovery

P 3.01 Structural-based computer-aided rational design of a ß-hairpin scaffold for BRCT-specific phosphorylated peptide binding
R.J.F. Branco, Í.L. Batalha, Universidade Nova de Lisboa, Caparica/P; O. Iranzo, Aix-Marseille Université/F; A.C.A. Roque, Universidade Nova de Lisboa, Caparica/P
P 3.02 Computer-aided design of high-affinity mutants of interferon-gamma receptor 1
B. Schneider, P. Mikulecký, J. Cerný, L. Biedermannová, P. Sebo, Institute of Biotechnology AS CR, Prague/CZ
P 3.03 Mutagenesis Objective Search and Selection Tool (MOSST): an algorithm to design protein affinity for specific ligands
A. Olivera-Nappa, J.A. Asenjo, B.A. Andrews, University of Chile, Santiago/RCH
P 3.04 Understanding the effect of agonist/antagonist binding in the activation/inactivation process of the serotonin 5HT6 receptor
N. Díaz-Vergara, A. Gonzalez, Universidad Autònoma de Barcelona, Bellaterra/E; T. de la Fuente, M.L. López-Rodríguez, Universidad Complutense de Madrid/E; L. Pardo, Universidad Autònoma de Barcelona, Bellaterra/E
P 3.05 Binding free energy, energy and entropy calculations using simple model systems
B. Lai, C. Oostenbrink, University of Natural Resources and Life Sciences, Vienna/A
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6. Nanotechnology, micro- & nanosystems

P 4.01 Microfluidic photosensor system for HIV-1 virion infectivity factor detection
C. Vistas, University of Algarve, Faro/P; J.P. Conde, INESC Microsystems and Nanotechnologies, Lisbon/P; G. Ferreira, University of Algarve, Faro/P
P 4.02 Invitro toxicity testing utilizing microfluidic affinity biosensors
G. Bergström, N. Robinson, C.-F. Mandenius, Linköping University/S
P 4.03 Gold derivatized nanocomposites for the effective detection, contrast imaging and thermal ablation in cancer research
R. Guzman, M. Garcia-Soto, University of Arizona, Tucson, AZ/USA
P 4.04 Synthesis and characterization of polymeric nanoparticle structures for control drug delivery in cancer therapies
R. Guzman, A. Lucero-Acuña, University of Arizona, Tucson, AZ/USA
P 4.05 Embedded microfluidic retroreflectors for sensitive pathogen diagnostics
B. Raja, C. Pascente, D. Shakarisaz, J. Knoop, T. Sherlock, A. Kar, K. Kourentzi, P. Ruchhoeft, University of Houston, TX/USA; J. Olano, University of Texas-Medical Branch, Galveston, TX/USA; A. Hatch, Sandia National Laboratories, Livermore, CA/USA; R.C. Willson, University of Houston, TX/USA
P 4.06 Poly(ethylene oxide)based heterobi-functional dendrimer-like polymers conjugated with bioacbioactive principle for targeted therapy
J. John, V. Kari, VIT University, Vellore/IND; Y. Gnanaou, KAUST, Thuwal/SAR; M.A. Vijayalakshmi, VIT University, Vellore/IND
Cancelled by the author

P 4.07 Miniaturization of capture assays for dengue detection in airports: a comparative evaluation of available technologies
J.R.C. Trabuco, Instituto Superior Técnico, Lisboa/P; R.C. Willson, University of Houston, TX/USA; D.M.F. Prazeres, Instituto Superior Técnico, Lisboa/P
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8. Biomimetics and evolutionary engineering of biomaterials

P 5.01 Biomimetic ligands for the profiling of clinically relevant proteins
Í.L. Batalha, Universidade Nova de Lisboa, Monte de Caparica/P; C.R. Lowe, University of Cambridge/UK; A.C.A. Roque, Universidade Nova de Lisboa, Monte de Caparica/P
P 5.02 New cross linking strategies for multipurpose PVA hydrogels: from biocatalysts to biomedical devices
M.A. Nunes, P. Gois, University of Lisbon/P; P.C. Fernandes, Instituto Superior Técnico, Lisbon/P; M.H.L. Ribeiro, University of Lisbon/P
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9. Affinity technologies in bioprocessing and biopharmaceuticals development

P 6.01 PEGylation, separation and characterization of laccase from Trametes versicolor
K. Mayolo-Deloisa, M. Gonzalez, J. Simental, M. Rito-Palomares, Tecnologico de Monterrey/MEX
P 6.02 Affinity-based, quantitative proteomic analysis of pancreatic cancer
J. Hoheisel, C. Schröder, M.S.S. Alhamdani, A.S. Bauer, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg/D; E. Costello, W. Greenhalf, J. Neoptolomos, Royal Liverpool University Hospital/UK; A. Scarpa, Verona University/I; N. Giese, J. Werner, M.W. Büchler, University of Heidelberg/D
P 6.03 Comparison of different affinity materials for the primary capture of antibodies
M. Freiherr von Roman, L. Janoschek, S. Berensmeier, TU München, Garching/D
P 6.04 A semi - empirical model as a tool to predict biomolecular partitioning in aqueous two phase system
D.P.C. de Barros, Instituto Superior Técnico, Lisbon/P; S.R.R. Campos, Instituto de Tecnologia Química e Biológica, Oeiras/P; P.P. Madeira, Universidade do Porto/P; A.M. Azevedo, Instituto Superior Técnico, Lisbon/P; A.M. Baptista, Instituto de Tecnologia Química e Biológica, Oeiras/P; M.R. Aires-Barros, Instituto Superior Técnico, Lisbon/P
P 6.05 Phenylboronic acid as ligand for a multimodal chromatography: characterization using protein library
R.Jr. Carvalho, Instituto Superior Técnico, Lisbon/P and Rensselear Polytechnic Institute, Troy, NY/USA; J. Woo, Rensselear Polytechnic Institute, Troy, NY/USA; A.M. Azevedo, Instituto Superior Técnico, Lisbon/P; S.M. Cramer, Rensselear Polytechnic Institute, Troy, NY/USA; M.R. Aires-Barros, Instituto Superior Técnico, Lisbon/P
P 6.06 A process for next generation antibody production: alternative methods to purify antibodies
A. Tscheliessnig, P. Satzer, N. Hammerschmid, University of Natural Resources and Life Sciences, Vienna/A; H. Schulz, B. Helk, Novartis International AG, Basel/CH; A. Jungbauer, University of Natural Resources and Life Sciences, Vienna/A
P 6.07 FRAP as biophysical tool to probe RNA and pDNA interaction with chromatographic ligands in membrane adsorbers
M.E. Monteiro, L. Raiado-Pereira, D.M.F. Prazeres, M. Mateus, Instituto Superior Técnico, Lisbon/P
P 6.08 Affinity molecules for site specific covalent labelling and detection
S. Kanje, A. Konrad, S. Hober, Royal Institute of Technology (KTH), Stockholm/S
P 6.09 Separation and characterization of antibody isoforms using electrophoretic methods
B. Hintersteiner, University of Natural Resources and Applied Life Sciences, Vienna/A; M. Schuster, APEIRON Biologics AG, Vienna/A; A. Jungbauer, University of Natural Resources and Applied Life Sciences, Vienna/A
P 6.10 Chromatographic characterization of immunoglobulin G
N. Lingg, University of Natural Resources and Life Sciences, Vienna/A; M. Bardor, Bioprocessing Technology Institute, Singapore/SGP; A. Jungbauer, University of Natural Resources and Life Sciences, Vienna/A
P 6.11 Affinity tags for one-step protein purification employing fluorapatite chromatography
T. Islam, N.S. Bibi, R.R. Vennapusa, R.N. D'Souza, Jacobs University of Bremen/D; J.M. Aguilar-Yañez, M. Rito-Palomares, Tecnológico de Monterrey/MEX; M. Fernandez-Lahore, Jacobs University of Bremen/D
P 6.12 Modified magnetic particles coated with aminophenyl boronic acid for antibody purification
V. Dhadge, Universidade Nova de Lisboa, Monte de Caparica/P; A. Azevedo, Instituto Superior Técnico, Lisbon/P; A. Hussain, Universidade Nova de Lisboa, Monte de Caparica/P; R. Aires-Barros, Instituto Superior Técnico, Lisbon/P; A.C.A. Roque, Universidade Nova de Lisboa, Monte de Caparica/P
P 6.13 Autodisplay of biotin-binding streptavidin
M. Park, University of Münster/D; J.H. Bong, Yonsei University, Seoul/ROK; J. Jose, University of Münster/D; J.C. Pyun, Yonsei University, Seoul/ROK
Cancelled by the author

P 6.14 Batch adsorption analysis of small proteins on a controlled access polymer permeation barrier to affinity binding sites of immobilized metal ions
R. Guzman, University of Arizona, Tucson, AZ/USA; O. Gonzalez-Ortega, University of Saint Luis Potosi/MEX; J. Porath, Uppsala University/S
P 6.15 Phenylboronic acid as ligand for a multimodal chromatography: proof of concept
R. Carvalho, Instituto Superior Técnico, Lisbon/P and Rensselear Polytechnic Institute, Troy, NY/USA; J. Woo, S. Parimal, Rensselear Polytechnic Institute, Troy, NY/USA; M.R. Aires-Barros, Instituto Superior Técnico, Lisbon/P; S.M. Cramer, Rensselear Polytechnic Institute, Troy, NY/USA; A.M. Azevedo, Instituto Superior Técnico, Lisbon/P
P 6.16 Sophorolipids: development of novel microbial glycolipids with cytotoxic activity towards MDA-MB-231 breast cancer cells
I.A.C. Ribeiro, C.M.C. Faustino, R.F.M. Frade, University of Lisbon/P; M.R. Bronze, University of Lisbon/P and Instituto de Tecnologia Química e Biológica, Oeiras/P; M.F. Castro, M.H.L. Ribeiro, University of Lisbon/P
P 6.17 New IMAC media enabling purification of histidine-tagged proteins directly from eukaryotic cell culture supernatants
T. Granér, M. Algotsson, L.C. Andersson, M. Björner, M. Carlsson, G. Glad, H. Hedlund, L. Hörnsten, P. Le Greves, H. Lindgren, S. Lindqvist, K. Öberg, GE Healthcare, Uppsala/S; L. Mathe, GE Healthcare Europe GmbH, München/D
P 6.18 Use of monolithic columns with immobilized gold nanoparticles for the separation of SH protein variants
C. Walther, Austrian Centre of Industrial Biotechnology, Vienna/A; A. Jungbauer, University of Natural Resources and Life Sciences Vienna, Vienna/A; Y. Lu, F. Svec, The Molecular Foundry, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA/USA
P 6.19 Affinity-ultrafiltration revisited
H. Filippusson, University of Iceland, Reykjavík/IS
P 6.20 AFFINImeter: a new tool to analyze isothermal titration calorimetry measurements
N. Díaz-Vergara, Universidad Autònoma de Barcelona, Bellaterra/E; L. Raposo, L. Sabín, A. Piñeiro, Universidad de Santiago de Compostela/E
P 6.21 Method to determine the surface energy of radial chromatography columns
I. Bednar, R. Tscheließnig, E. Berger, Austrian Centre of Industrial Biotechnology (ACIB), Vienna/A; N. Lendero Krajnc, A. Podgornik, BIA Separations, Ajdovšcina/SLO; A. Jungbauer, Austrian Centre of Industrial Biotechnology (ACIB), Vienna/A; A. Jungbauer, University of Natural Resources and Life Sciences Vienna, Vienna/A
P 6.22 Immobilization-stabilization of imibond ß-galactosidase from Kluyveromyces lactis
B. Franco, M. Nunes, M.H.L. Ribeiro, University of Lisbon/P
P 6.23 Novel magnetic cross-linked enzyme aggregates (mCLEAs) of naringinase
S. Martins, M. Nunes, M.H.L. Ribeiro, University of Lisbon/P
P 6.24 Selective biopurification utilizing multimodal interaction principles
C. Voß, Bio-Rad Laboratories GmbH, München/D; X. He, Bio-Rad Laboratories Inc., Hercules, CA/USA
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10. Affinity in biomaterials and biosensors

P 7.01 Bioprocessing different targeted cytokines for biopharmaceutical development to treat different cancer entities
N. Klehr, B. Brüderl, L. Bauer, J. Setter, S. Sowa, Haemotec GmbH, Grünwald/D
P 7.02 Protein-DNA interaction analysis using acoustic wave sensors
R.M.M. Rodrigues, J. de-Carvalho, B. Tome, G.N.M. Ferreira, University of Algarve, Faro/P
P 7.03 Flow cytometric immunoassay using autodisplay of high affinity protein
M. Park, University of Münster/D; J.H. Bong, Yonsei University, Seoul/ROK; J. Jose, University of Münster/D; J.C. Pyun, Yonsei University, Seoul/ROK
Cancelled by the author

P 7.04 Affinity tagged plasmonic and polymeric nanoparticles for cancer theraphy and imaging diagnostics
R. Guzman, A. Lucero-Acuña, M. Garcia-Soto, University of Arizona, Tucson, AZ/USA
P 7.05 Towards biotinyl moiety selective synthetic polymers though ultra thin films and hierarchical imprinting strategies
S. Suriyanarayanan, L. Elmlund, J.G. Wiklander, I.A. Nicholls, Linnaeus Univeristy, Kalmar/S
P 7.06 Polyelectrolyte multilayers as affinity carriers for growth factors in designing bioactive biomaterial surfaces
H.P. Jennissen, S. Madenci, University of Duisburg-Essen, Essen/D; S. Lüers, M. Laub, Morphoplant GmbH, Bochum/D
P 7.07 Remote-controlled vaccine depots based on the selective interaction of proteins to small-molecules
R.J. Gübeli, D. Hövermann, University of Freiburg/D; H. Seitz, M. Müller, German Cancer Research Center (DKFZ), Heidelberg/D; W. Weber, University of Freiburg/D
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