3D Cell Culture 2021: Models, Applications & Translation

Lecture Programme

(subject to change, as of 30 April 2021)

Wednesday, 5 May 2021

Thursday, 6 May 2021

Friday, 7 May 2021

Programme PDF Download

Wednesday, 5 May 2021

Opening and Technical Remarks
Synthetic Biology, Screening Platforms and Metabolomics

Chair: H. Hauser, Helmholtz Centre for Infection Research, Braunschweig/D

Keynote Lecture 1
Toward a world of ElectroGenetics
M. Fussenegger¹; ¹ ETH Zurich/CH
10:40 Advanced in vitro management of three-dimensional cell cultures and explanted tissue
S. Kreß¹; D. Egger¹; C. Kasper¹
¹ University of Natural Resources and Life Sciences (BOKU), Vienna/A

Label-free measurements of the metabolic activity within 3D cell culture model via automated 3D microphysiometry
S. Eggert¹; M. Gutbrod²; G. Liebsch²; R. Meier²; D. Hutmacher³; P. Mela¹
¹ Technical University of Munich, Garching/D; ² PreSens Precision Sensing GmbH, Regensburg/D; ³ Queensland University of Technology, Brisbane/AUS



Hypoxia or normoxia: mesenchymal stem cells and chondrocytes with a genetically integrated hypoxia sensor in different 3D cell culture systems
C. Schmitz¹; T. Fleischhammer¹; I. Pepelanova¹; E. Potekhina²; V. Belousov³;
T. Scheper¹; A. Lavrentieva¹
¹ Leibniz University Hanover/D; ² Shemyakin‐Ovchinnikov Institute of Bioorganic Chemistry, Moscow/RUS; ³ Federal Center of Brain Research and Neurotechnologies, Federal Medical Biological Agency, Moscow/RUS


EU-OPENSCREEN: A novel collaborative model for accelerating early phase drug discovery
A. Silvestri¹; O. Genilloud²; P. Gribbon³; J. Kolanowski⁴; Z. Leśnikowski⁵;
C. Steinhauer⁶; M. Vicent⁷; W. Fecke¹
¹ EU-OPENSCREEN ERIC, Berlin/D; ² Fundación Medina, Granada/E;
³ Fraunhofer Institute for Molecular Biology and Applied Ecology, Hamburg/D;
⁴ Polish Academy of Sciences, Poznan/PL; ⁵ Polish Academy of Sciences, Lodz/PL;
⁶ Copenhagen University/DK; ⁷ Principe Felipe Research Center, Valencia/E

11:30 Discussion with all speakers of this session
11:45 Lunch Break
Advanced 3D Models for Tumor Research

Chair: J.M. Kelm, PreComb Therapeutics AG, Wädenswil/CH

Keynote Lecture 2
Organotypic 3D models to characterize the molecular requirements of immune cell infiltration and activation
W. Sommergruber¹; A. Osswald²; I. Voelkl³; V. Hedrich³; A. Rudisch³; N. Harrer³;
M. Dong⁴; H. van der Kuip⁴; A. Wernitznig³; A. Bernthaler³; J. Lipp³; S. Rebelo⁵;
C. Pinto³; C. Brito⁵; S. Hall⁶
¹ University of Applied Sciences Vienna/A; ² Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach/D; ³ Boehringer Ingelheim RCV GmbH & Co KG, Vienna/A;
⁴ University of Tuebingen/D; ⁵ iBET, Universidede Nova de Lisboa/P; ⁶ University Hospital Bern/CH

Development of a 4D-in vitro-test system for specificity and potency of 3rd generation chimeric antigen receptor-T-cells (CAR-T-cells) based on microcavity array-bioreactors
E. Gottwald¹; L. Werner¹; C. Nies¹; S. Wang²; M. Schmitt²
¹ Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen/D;

² University Hospital Heidelberg/D


3D modelling of the chronic lymphocytic leukaemia microenvironment
F. Sbrana¹; R. Pinos¹; D. Ribezzi¹; F. Scagnoli¹; F. Barbaglio¹; D. Belloni¹;
L. Scarfò¹; C. Scielzo¹
¹ IRCCS, Ospedale San Raffaele, Milan/I

 14:30 Targeting the fibrotic tumor microenvironment: Novel 3D co-culture organoids for pancreatic adenocarcinoma (PDAC) drug discovery
J. Hänsel¹; K. Peter¹; T. Kurz²; R. Mrsny³; N. Teusch¹
¹ Universität Osnabrück/D; ² Heinrich-Heine-Universität Düsseldorf/D; ³ University of Bath/UK


Generation of multicellular spheroid culture models of pediatric vital tumor samples obtained through the INFORM registry study for functional studies
H. Peterziel¹; A. Mangang¹; P. Fiesel²; S. Oppermann³; L. Turunen⁴; J. Saarela⁴;
O. Witt⁵; I. Oehme¹
¹ Hopp Children’s Cancer Center Heidelberg (KiTZ), German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK) Heidelberg/D; ² German Cancer Research Center (DKFZ) and German Consortium for Translational Cancer Research (DKTK) Heidelberg/D; ³ Hopp Children’s Cancer Center Heidelberg (KiTZ), German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg/D; ⁴ University of Helsinki, Finland (FIMM-UH), Helsinki/FIN; ⁵ Hopp Children’s Cancer Center Heidelberg (KiTZ), German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK) and Heidelberg University Hospital/D

14:50 Discussion with all speakers of this session
Poster Session 1 / Exhibition

End of Day 1



Section Member Assembly

DECHEMA Sections "Cell Culture Technology" and "Medical Biotechnology"

 Thursday, 6 May 2021

Complex and Multi-Celltype Models

Chair: C. Kasper, University of Natural Resources and Life Sciences, Vienna/A


Image based quantification of myeloid cell repolarization and their interplay within the tumor microenvironment in 3D
G. Goverse¹; N. Beztsinna¹; B. Visser¹; M. van de Merbel¹; E. Spanjaard¹; K. Yan¹;
L. Price¹; L. Daszkiewicz¹
¹ OcellO B.V., Leiden/NL

09:15 Application of ultrasmall gold nanoparticles (2 nm) in a 3D blood-brain-barrier in vitro model
V. Sokolova¹; G. Mekky²; S. van der Meer¹; M. Seeds³; A. Atala³; M. Epple¹
¹ University of Duisburg-Essen, Essen/D; ² Zagazig University/ET; ³ Wake Forest School of Medicine, Winston-Salem, NC/USA

Manufacturing of functional beta cell-mesenchymal stem cell-spheroids for transplantation or drug testing
F. Petry¹; P. Czermak¹,²; D. Salzig¹
¹ University of Applied Sciences Mittelhessen, Giessen/D

² University of Giessen/D

09:45 Assembly of multi-spheroid cellular architectures by programmable droplet merging
H. Cui¹; A. Popova¹; P. Levkin¹
¹ Karlsruher Institut für Technologie (KIT), Eggenstein-Leopoldshafen/D


Development of a new human organotypic 3D urinary bladder epithelium model in complex whole blood co-culture with immune cells
M. Willig¹; R. Kehlbach¹; G. Stein¹; M. Schmolz¹
¹ HOT Screen GmbH, Reutlingen/D


Discussion with all speakers of this session


Coffee Break

Innovative Microphysiological Systems I

Chair: U. Marx, TissUse GmbH, Berlin/D

Keynote Lecture 3
Application of microphysiological systems to drug safety assessment: progress and challenges
R. David¹; ¹ AstraZeneca, Cambridge/UK
11:30 OncoChip - A novel microfluidic platform for compound efficacy testing on human 3D microtumors
M. Pawlak¹; S. Yüz²; B. Gierke¹; J. Hofmann³; U. Härle²; S. Werner²;
B. Hagmeyer²; Z. von Guttenberg³; M. Stelzle²; C. Schmees²
¹ NMI TT Pharmaservices, Reutlingen/D; ² NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen/D; ³ ibidi GmbH, Gräfelfing/D


Applying a microphysiological 3D human liver – islet microtissue platform to study drug – drug interaction
L. Hoelting¹; I. Karakoc¹; B. Yesildag¹; W. Moritz¹; O. Frey¹
¹ InSphero AG, Schlieren/CH


Evaluating drug-induced liver toxicity of acetaminophen, trovafloxacin and levofloxacin in a triple-cell microphysiological liver sinusoidal model

T. Kaden¹; R. Li²; A. Mosig³; K. Graf¹; M. Raasch¹; K. Rennert¹
¹ Dynamic42 GmbH, Jena/D; ² Biopredic sarl, St Gregoire/F; ³ University Hospital Jena/D


Discussion with all speakers of this session


Lunch Break

Poster Session 2 / exhibition
Innovative microphysiological systems II

Chair: J. Hansmann, Universitätsklinikum Würzburg/D

Keynote Lecture 4
Advancing regulatory science through innovation; in vitro microphysiological systems
S. Fitzpatrick¹
¹ Center for Food Safety and Applied Nutrition / FDA, College Park, MD/USA
15:30 A human immuno-competent microphysiological alveolus-on-chip model to study microbial pathogenesis
K. Rennert¹; S. Deinhardt-Emmer²; E. Schicke²; Z. Cseresnyés³; S. Nietzsche²;
M. Raasch¹; G. Makert dos Santos⁴; S. Ulbert⁴; P. Loskill⁵; C. Ehrhardt²; B. Löffler²;
A. Mosig²
¹ Dynamic42 GmbH, Jena/D; ² University Hospital Jena/D; ³ Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena/D;
⁴ Fraunhofer Institute for Cell Therapy and Immunology, Leipzig/D; ⁵ Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart/D
15:45 Micro-physiological system for prolonged cultivation of immune competent lung tissue ex vivo
S. Böhlen¹; S. Konzok¹; S. Behrens²; F. Schmieder²; F. Sonntag²; A. Braun¹;
S. Dehmel¹; K. Sewald¹
¹ Fraunhofer Institute for Toxicology and Experimental Medicine ITEM & Biomedical Research in Endstage and Obstructive Lung Disease Hanover (BREATH), Hanover/D;
² Fraunhofer Institute for Material and Beam Technology IWS Dresden/D


Development of vascularized melanoma skin equivalents for studying metastasis and anti-melanoma therapies
A. Leikeim¹; J. Kliche¹; M. Komma¹; F. Schmidt²; F. Groeber-Becker²
¹ Uniklinikum Würzburg/D; ² Fraunhofer ISC - Translational Center Regenerative Therapies TLC-RT, Würzburg/D


Discussion with all speakers of this session

Award Ceremony
Virtual Get Together at "wonder"

End of Day 2

 Friday, 7 May 2021

Investigating Host-Microbial Interactions

Chair: I. Prade, Forschungsinstitut für Leder und Kunststoffbahnen (FILK) gGmbH, Freiberg/D

09:00 Microengineered in vitro model of the small intestine epithelium for studying host-bacteria interactions
M. García-Díaz¹; M. Cendra¹; R. Alonso-Román¹; E. Torrents¹; E. Martínez¹
¹ Institute for Bioengineering of Catalonia (IBEC), Barcelona/E
09:15 Matrix-free culture of intestinal organoids in thermoformed microwells
P. Kakni¹; S. Giselbrecht¹; R. Truckenmüller¹; P. Habibovic¹
¹ Maastricht University/NL
09:30 Investigating human cytomegalovirus infection in human vessels based on novel in vitro and in vivo model systems
A. Dittrich¹; I. Kutle¹; D. Wirth¹
¹ Helmholtz Centre for Infection Research, Braunschweig/D

Discussion with all speakers of this session


Coffee Break

Bioprinting and Enabling Technologies
Chair: M. Rimann, Zurich University of Applied Sciences, Wädenswil/CH
10:30 Fabrication of human jawbone models using stereolithographic 3D bioprinting
A. Amler¹; A. Thomas¹; T. Lam²; M. Geiger²; A. Kreuder¹; C. Palmer²; S. Nahles³;
R. Lauster¹; L. Kloke²
¹ TU Berlin/D; ² Cellbricks GmbH, Berlin/D; ³ Charite Universitätsmedizin Berlin/D
10:45 Drop-on-demand bioprinting solutions for the fabrication of 3D cell culture systems aiming for in vitro screening applications
K. Tröndle¹; A. Itani¹; F. Koch¹; R. Zengerle²; P. Koltay¹; S. Zimmermann¹
¹ University of Freiburg/D; ² Hahn-Schickard, Freiburg/D
11:00 Using peptide hydrogels to build 3D cell disease and tissue models for drug discovery
A. Miller¹; ¹ University of Manchester/UK
11:15 Discussion with all speakers of this session
11:30 Closing Remarks
11:35 End of the Conference
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